Brand new innovative Products


Serum-free Systems

by PAN-Biotech

Address

Am Gewerbepark 6, D-94501 Aidenbach / Germany

Serum-free Systems

 

 

 

 

 

 

Advantages of serum-free cell culture

Lower risk

 

Lower risk of contamination with bacteria, fungi, mycoplasma or virus.

Reproducibility

 

Defined formulations result in more reproducible and consistent research data.

Time-saving


Time-consuming batch tests are no more necessary.

Ethic



No more animal experiments.

Easy

downstream

 

Ease of purification of end products.

Compliance

 

 

In compliance with pharmaceutical and clinical standards.
 

Flexibility



Reduce or replace serum in cell culture medium.

Only at PAN Biotech: Panexin

Fully defined serum replacements 

Panexin

No more serum testing!

 

Panexin products are fully defined serum replacements developed by PAN-Biotech. They can be used for the cultivation of adherent and non-adherent cells under serum-free or serum-reduced conditions. For more demanding cell lines we also designed Panexin NTA (adherent cells) and NTS (suspension cells).


Enjoy the reproducibility and the flexibility!

 

 

 

 

 

 

 

 

 

 

 

Applications


  As serum replacement or medium              supplement to increase the productivity in      industrial cell cultures (CHO, MDCK, Vero,    Hybridoma etc.)

  To avoid the exosomes or stimulatory        effects of growth factors in serum

  To prevent the overgrowth of the culture   by fibroblasts in coculture or in highly            differentiated epithelial primary cultures

  To guarantee the reproducibility and         sensitivity in cell-based in vitro assays.

  To generally reduce the amount of serum due to ethical concerns, lot-to-lot variability or high costs





Advantages

 

  No more serum testing

  High reproducibility

  Flexibility

  Reliability

  Cost efficiency

  Lower investment

  Simplified downstream

  GMP compliant

 

 

 

 

 

 

 

 

 

 

Do you know?


Serum introduces several severe unknown variables into the cell culture procedure, as serum

  (a) is a poorly defined supplement (Bjare,  1992; Gstraunthaler, 2003);

  (b) batches show typically qualitative          variations and different amount of                 endotoxins, haemoglobin and other               factors (Price and Gregory, 1982);

  (c) can be a potential source of                   contamination (Dormont, 1999; Eliot, 1999;  Wessman and Levings, 1999) and

  (d) does not represent physiological           conditions.

Therefore, FBS may alter the experimental output or the performance of assays.


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Panexin basic

Defiened Serum Replacement

Panexin basic

Panexin basic is designed to replace or to reduce serum in the cell culture in a very simple manner. In most cases there is no need to change the basal medium. As Panexin basic is fully defined and contains no peptones or hydrolysates, lot testing is not necessary anymore. It also allows high reproducibility and simplified downstream process. Panexin basic contains no growth factors and enables defined proliferation and differentiation of stem cells. Characterization studies of growth factors will obtain more reproducible and clearer results. Panexin basic is also useful to develop sensitive cell-based in vitro tests and coculture procedures. For cell lines which require specific growth factors these should be added in a concentration as previously used.

Composition

 

Panexin basic contains purified proteins, lipids, salts, amino acids, trace elements, hormones and a 3-dimensional substrate release system in an optimized formulation. It contains no growth factors, undefined hydrolysates or peptones.

 

 

 

 

Suitability

 

Panexin basic is suitable for the cultivation of a variety of adherent and non-adherent cells under serum-free culture conditions or to reduce the necessary FBS amount in cell culture. (see Fig.1)

 

 

 

 

 

 

Storage conditions

 

Storage: -20°C, in the dark

Stability: 2 years from date of production Size: 50 ml, 100 ml, 500 ml, other sizes on request.

 

 

 

 

 

 

 

Panexin CD

Chemically Defined Serum Replacement

Panexin CD

Panexin CD is designed to replace or to reduce serum in the cell culture in a very simple manner. Compared to other serum replacements, it contains only chemically defined components. Due to its very defined character, lot testing is not necessary anymore. It also allows high reproducibility and simplified downstream processing. Panexin CD contains no growth factors and enables defined proliferation and differentiation of stem cells. Characterization studies of growth factors will obtain more reproducible and clearer results. Panexin CD is also useful to develop sensitive cell-based in vitro tests and coculture procedures. For cell lines which require specific growth factors these should be added in a concentration as previously used

Composition

 

Panexin CD contains recombinant proteins, lipids, salts, amino acids, trace elements, hormones and a 3-dimensional substrate release system in an optimized formulation. It contains no growth factors, undefined hydrolysates or peptones. 

 

 

 

 

 

 

 

Suitability

 

Panexin CD is suitable for the cultivation of a variety of adherent and non-adherent cells under serum-free culture conditions or to reduce the necessary FBS amount in cell culture.

 

 

 

 

 

 

 

Storage conditions

 

Storage: -20°C, in the dark

Stability: 2 years from date of production Size: 50 ml, 100 ml, 500 ml, other sizes on request.

 

 

 

 

 

 

 

 

 

Panexin NTA

Defined Serum Substitute for Adherent Cells

Panexin NTA

Panexin NTA is designed to replace or to reduce serum in the cell culture in a very simple manner. In most cases there is no need to change the basal medium. As Panexin NTA is fully defined and contains no peptones or hydrolysates, lot testing is no more necessary. It also allows high reproducibility and a simplified downstream process. Panexin NTA contains no growth factors and enables defined proliferation and differentiation of stem cells. Characterization studies of growth factors will obtain more reproducible and clearer results. Panexin NTA is also useful to develop sensitive cell-based in vitro tests and coculture procedures. For cell lines which require specific growth factors these should be added in a concentration as previously used.

Composition


Panexin NTA contains purified proteins, lipids, salts, amino acids, trace elements, attachment factors and hormones in an optimized formulation. Panexin NTA contains no growth factors, undefined hydrolysates or peptones.






Suitability


Panexin NTA is suitable for the cultivation of a variety of adherent cells under serum-free culture conditions (please see figure 1) or to reduce the necessary FBS amount in cell culture.








Storage conditions

 

Storage: -20°C, in the dark

Stability: 2 years from date of production Size: 50 ml, 100 ml, 500 ml, other sizes on request.

 

 

 

 

 

 

 

 

 

Panexin NTS

Defined Serum Substitute for Suspension Cells

Panexin NTS

Panexin NTS is designed to replace or to reduce serum in the cell culture in a very simple manner. In most cases there is no need to change the basal medium. As Panexin NTS is fully defined and contains no peptones or hydrolysates, lot testing is no more necessary. It also allows high reproducibility and a simplified downstream process. Panexin NTS contains no growth factors and enables defined proliferation and differentiation of stem cells. Characterization studies of growth factors will obtain more reproducible and clearer results. Panexin NTS is also useful to develop sensitive cell-based in vitro tests and coculture procedures. For cell lines which require specific growth factors these should be added in a concentration as previously used.

Composition


Panexin NTS contains purified proteins, lipids, salts, amino acids, trace elements, and hormones in an optimized formulation and a new 3-dimensional substance release system (3D-SRS). Panexin NTS contains no growth factors, undefined hydrolysates or peptones







Suitability

 

Panexin NTS is suitable for the cultivation of a variety of non-adherent cells under serum-free culture conditions (please see figure 1 for examples) or to reduce the necessary FBS amount in cell culture.

 

 

 

 

 

 

 

Storage conditions

 

Storage: -20°C, in the dark

Stability: 2 years from date of production Size: 50 ml, 100 ml, 500 ml, other sizes on request.

 

 

 

 

 

 

 

 

 

Panexin BMM

Serum Substitute for Cultivation of Mouse Bone Marrow Macrophages (BMM)

Panexin BMM

Panexin BMM is a fully defined serum substitute for the cultivation of macrophages from mouse bone marrow (murine bone marrow derived macrophages, BMM) under serum-free conditions. Panexin BMM is added to the basal medium RPMI 1640 in a final concentration of 5 % and has to be supplemented with 50 μM Mercaptoethanol and 2 ng/ml GM-CSF murine rec.

Panexin BMM has been developed for the generation of macrophages from mouse bone marrow under serum-free conditions. The results will be more comparable, as undefined components - like in serum-containing cultures - are eliminated. In Panexin BMM matured macrophages will show excellent attachment capabilities. This achieves standardized conditions and reproducible results.

Composition

 

Panexin BMM contains purified proteins, lipids, salts, amino acids, trace elements, attachment factorsand hormones in an optimized formulation. It contains no growth factors, undefined hydrolysates or peptones.

 

 

 

 

 

 

 

 

Reference

 

Kristin Eske, Generation of murine bone marrow derived macrophages in a standardized serum-free cell culture system. Journal of Immunological Methods 342 (2009) 13–19.

 

 

 

 

 

 

Storage conditions

 

Storage: -20°C, in the dark

Stability: 2 years from date of production Size: 10 ml, 100 ml, other sizes on request.

 

 

 

 

 

 

 

 

Tables

Overview

Tables

PAN-Biotech

Your expert in cell culture

Company profile

Since 1988 PAN-Biotech has demonstrated its strength with innovative, first-class quality products and excellent services for cell culture around the globe.

Specialized in the manufacture and distribution of cell culture products like sera, media, serum-free systems, reagents, biologicals and more we deliver our goods to customers from research, clinics and the biopharmaceutical industry.

Our highly skilled team with operating experience in various fields of cell technology guarantees product development based on the needs and requirements of our customers. Individual wishes are always welcome.


Production takes place in Germany, Aidenbach, Bavaria.

Quality: "Made in Germany"! at our Medical Clinic centres on diagnosing and treating patients of all ages, while emphasizing preventative medicine and the overall health and wellness of its patients.