Serum-free Systems

Applications

As serum replacement or medium supplement to increase the productivity in industrial cell cultures (CHO, MDCK, Vero, Hybridoma etc.)

To avoid the exosomes or stimulatory effects of growth factors in serum

To prevent the overgrowth of the culture by fibroblasts in coculture or in highly differentiated epithelial primary cultures

To guarantee the reproducibility and sensitivity in cell-based in vitro assays.

To generally reduce the amount of serum due to ethical concerns, lot-to-lot variability or high costs

Do you know?

Serum introduces several severe unknown variables into the cell culture procedure, as serum

(a) is a poorly defined supplement (Bjare, 1992; Gstraunthaler, 2003);

(b) batches show typically qualitative variations and different amount of endotoxins, haemoglobin and other factors (Price and Gregory, 1982);

(c) can be a potential source of contamination (Dormont, 1999; Eliot, 1999; Wessman and Levings, 1999) and

(d) does not represent physiological conditions.

Therefore, FBS may alter the experimental output or the performance of assays.

Panexin basic is designed to replace or to reduce serum in the cell culture in a very simple manner. In most cases there is no need to change the basal medium. As Panexin basic is fully defined and contains no peptones or hydrolysates, lot testing is not necessary anymore. It also allows high reproducibility and simplified downstream process. Panexin basic contains no growth factors and enables defined proliferation and differentiation of stem cells. Characterization studies of growth factors will obtain more reproducible and clearer results. Panexin basic is also useful to develop sensitive cell-based in vitro tests and coculture procedures. For cell lines which require specific growth factors these should be added in a concentration as previously used.

Panexin CD is designed to replace or to reduce serum in the cell culture in a very simple manner. Compared to other serum replacements, it contains only chemically defined components. Due to its very defined character, lot testing is not necessary anymore. It also allows high reproducibility and simplified downstream processing. Panexin CD contains no growth factors and enables defined proliferation and differentiation of stem cells. Characterization studies of growth factors will obtain more reproducible and clearer results. Panexin CD is also useful to develop sensitive cell-based in vitro tests and coculture procedures. For cell lines which require specific growth factors these should be added in a concentration as previously used

Panexin NTA is designed to replace or to reduce serum in the cell culture in a very simple manner. In most cases there is no need to change the basal medium. As Panexin NTA is fully defined and contains no peptones or hydrolysates, lot testing is no more necessary. It also allows high reproducibility and a simplified downstream process. Panexin NTA contains no growth factors and enables defined proliferation and differentiation of stem cells. Characterization studies of growth factors will obtain more reproducible and clearer results. Panexin NTA is also useful to develop sensitive cell-based in vitro tests and coculture procedures. For cell lines which require specific growth factors these should be added in a concentration as previously used.

Panexin NTS is designed to replace or to reduce serum in the cell culture in a very simple manner. In most cases there is no need to change the basal medium. As Panexin NTS is fully defined and contains no peptones or hydrolysates, lot testing is no more necessary. It also allows high reproducibility and a simplified downstream process. Panexin NTS contains no growth factors and enables defined proliferation and differentiation of stem cells. Characterization studies of growth factors will obtain more reproducible and clearer results. Panexin NTS is also useful to develop sensitive cell-based in vitro tests and coculture procedures. For cell lines which require specific growth factors these should be added in a concentration as previously used.

Panexin BMM is a fully defined serum substitute for the cultivation of macrophages from mouse bone marrow (murine bone marrow derived macrophages, BMM) under serum-free conditions. Panexin BMM is added to the basal medium RPMI 1640 in a final concentration of 5 % and has to be supplemented with 50 μM Mercaptoethanol and 2 ng/ml GM-CSF murine rec.

Panexin BMM has been developed for the generation of macrophages from mouse bone marrow under serum-free conditions. The results will be more comparable, as undefined components - like in serum-containing cultures - are eliminated. In Panexin BMM matured macrophages will show excellent attachment capabilities. This achieves standardized conditions and reproducible results.

Since 1988 PAN-Biotech has demonstrated its strength with innovative, first-class quality products and excellent services for cell culture around the globe.

Specialized in the manufacture and distribution of cell culture products like sera, media, serum-free systems, reagents, biologicals and more we deliver our goods to customers from research, clinics and the biopharmaceutical industry.

Our highly skilled team with operating experience in various fields of cell technology guarantees product development based on the needs and requirements of our customers. Individual wishes are always welcome.

Production takes place in Germany, Aidenbach, Bavaria.

Quality: "Made in Germany"! at our Medical Clinic centres on diagnosing and treating patients of all ages, while emphasizing preventative medicine and the overall health and wellness of its patients.

Serum-free Systems

by PAN-Biotech